A study of the antimicrobial efficancy of silk suture

  • Sibanda Pethile National University of Science and Technology, Bulawayo, Zimbabwe
  • Ncube Sizo
  • Ndlovu Lloyd
Keywords: Silk suture, antimicrobial, scouring, tenacity, surface morphological analysis

Abstract

In recent years many antimicrobial sutures have been developed in a quest to deal with the problem of surgical site infections in the medical world. This study was done to find out the effects of pre-treating the braided suture materials before applying the antibacterial coating agent. 0.1N sodium hydroxide (NaOH) solution was used to pre-treat / pre scour the sutures to remove impurities and also to open the structure of the silk sutures so as to allow the coating agent to penetrate into the suture structure. A biodegradable polymer and an antibacterial drug, that is, polycaprolactone (PCL) and Sulphamethoxazole trimethropm (SMZ) were used as the coating agent. The polymer PCL was made into a solution of 10% whilst the drug SMZ was made into a solution of 2500µg/ml. 5cm pieces of silk braided sutures were first cut aseptically and then washed in 0.1N NaOH solution and dried. After this pretreatment, they were coated with the antimicrobial coating agent and then dried. The pretreated sutures were then evaluated and compared, with non-treated sutures acting as the control. Antimicrobial tests were then carried out to find out the effects of pre-treated and non-pre-treated sutures on the antimicrobial properties of the sutures. Through this study it was found that the pre-treated sutures exerted a better sustained efficacy assay compared to those that are not pretreated, however mechanical properties were lowered but were still within the required standards for antimicrobial sutures.

References

Farid Saleh, B. P., Danielle Lodi,and Khalid Al- Sebeih (2008). International Journal of Medical Sciences 5(6): 354-360.

Alexander,J. W., J. Z. Kaplan and W. A. Altemeier (1967). Annals of surgery 165(2): 192-199.

Rodeheaver, G. T., L. D. Kurtz, W. T. Bellamy, S. L. Smith, H. Farris and R. F. Edlich (1983). "Biocidal braided sutures." Archives of surgery (Chicago, Ill. : 1960) 118(3): 322-327.

Uff, C. R., A. D. Scott, A. G. Pockley and R. K. S. Phillips (1995). Biomaterials 16(5): 355-360.

Liu, H., K. K. Leonas and Y. Zhao (2010). Journal of Engineered Fibers and Fabrics 5(4 ): 10-19.

Altman, G. H., F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. S. Chen, H. Lu, J. Richmond and D. L. Kaplan (2003). Biomaterials 24(3): 401-416.

Vepari, C. and D. L. Kaplan (2007). Progress in Polymer Science 32(8-9): 991-1007.

Vila, J., A. Soriano and J. Mensa (2008). EnfermedadesInfecciosas y MicrobiologíaClínica 26(1): 48-55.

Gao, G., D. Lange, K. Hilpert, J. Kindrachuk, Y. Zou, J. T. J. Cheng, M. Kazemzadeh-Narbat, K. Yu, R. Wang, S. K. Straus, D. E. Brooks, B. H. Chew, R. E. W. Hancock and J. N. Kizhakkedathu (2011). Biomaterials 32(16): 3899-3909.

Viju, S. and G. Thilagavathi (2013). Journal of Industrial Textiles 42(3): 256-268.

Ming, X., S. Rothenburger and M. M. Nichols (2008). Surgical Infections 9(4): 451-457.

Janiga, P. K., B. Elayarajah, R. Rajendran, R. Rammohan, B. Venkatrajah and S. Asa (2012). Journal of Industrial Textiles 42(2): 176-190.

Lou, C.-W., C.-H. Yao, Y.-S. Chen, T.-C. Hsieh, J.-H. Lin and W.-H. Hsing (2008). Textile Research Journal 78(11): 958-965.

Masini, B. D., D. J. Stinner, S. M. Waterman and J. C. Wenke (2011). Journal of Surgical Education 68(2): 101-104.

Elayarajah B, Rajendran R and Venkatrajah B (2011). International Journal of Pharmaceutical Sciences Review and Research 7(2): 336–343.

Singh, R., A. Jain, S. Panwar, D. Gupta and S. K. Khare (2005). Dyes and Pigments 66(2): 99-102.

Pinho, E., L. Magalhaes, M. Henriques and R. Oliveira (2011). Annals of Microbiology 61(3): 493-498.

KimiranErdem, A., SanliYurudu, N. O. (2008). IUFS Journal of Biology 67(2): 115-122.

Ming, X., S. Rothenburger and D. Yang (2007). Surgical Infections 8(2): 201-208.

Published
2019-06-06
Issue
Vol. 1 No. 1 (2019)
Section
Articles
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